Journal: World Journal of Stem Cells

Article Title: MicroRNA-584-5p/RUNX family transcription factor 2 axis mediates hypoxia-induced osteogenic differentiation of periosteal stem cells

doi: 10.4252/wjsc.v15.i10.979

Figure Lengend Snippet: Prediction of the miRNA-584-5p target. A: Potential binding sites of miRNA-584-5p and the 3’-untranslated region of RUNX family transcription factor 2 (RUNX2); B: Dual luciferase reporter gene assay; C: Quantitative polymerase chain reaction determination of RUNX2 expression; D: Western blot determination of RUNX2 protein expression. One-way ANOVA and t tests were used to demonstrate differences in RUNX2 responses between the miR584-5p mimic and negative control groups. a P < 0.05; b P < 0.01. RUNX2: RUNX family transcription factor 2; NC: Negative control; wt: Wild type; mut: Mutant; miRNA: MicroRNA.

Article Snippet: RNA was reverse-transcribed into cDNA using a reverse transcription-fluorescence quantitative polymerase chain reaction (qPCR) kit (Tiangen, Beijing, China) and PCR apparatus (Applied Biosystems, United States) for real-time fluorescence quantification (qPCR) after determining the purity of the RNA samples.

Techniques: Binding Assay, Luciferase, Reporter Gene Assay, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Negative Control, Mutagenesis

Journal: Experimental and Therapeutic Medicine

Article Title: Clinical characteristics and association analysis of persistent low-level HBsAg expression in a physical examination population with HBV infection

doi: 10.3892/etm.2019.8217

Figure Lengend Snippet: Distribution of HBsAg-positive prevalence in all 45,256 individuals and in 2,544 HBsAg-positive subjects.

Article Snippet: A total of 100 μl of eluent (i.e., HBV DNA extract) was collected for real-time quantitative fluorometric detection of HBV DNA, according to the manufacturer's protocol of HBV DNA fluorescence quantitative detection reagent kit [ACON Biotech (Hangzhou) co., Ltd.] and an ABI StepOnePlusTM real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.).

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Clinical characteristics and association analysis of persistent low-level HBsAg expression in a physical examination population with HBV infection

doi: 10.3892/etm.2019.8217

Figure Lengend Snippet: Clinical laboratory parameters of 2,544 HBsAg-positive subjects with HBV infection.

Article Snippet: A total of 100 μl of eluent (i.e., HBV DNA extract) was collected for real-time quantitative fluorometric detection of HBV DNA, according to the manufacturer's protocol of HBV DNA fluorescence quantitative detection reagent kit [ACON Biotech (Hangzhou) co., Ltd.] and an ABI StepOnePlusTM real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.).

Techniques: Infection

Journal: Experimental and Therapeutic Medicine

Article Title: Clinical characteristics and association analysis of persistent low-level HBsAg expression in a physical examination population with HBV infection

doi: 10.3892/etm.2019.8217

Figure Lengend Snippet: Distribution of the prevalence of different level HBsAg positivity and HBV DNA positivity in HBsAg-level groups according to age in a physical examination population. (A) Prevalence of HBsAg positivity in the low-level HBsAg group according to age in a physical examination population. (B) Prevalence of different levels of HBsAg positivity in the high-level HBsAg groups (≥10-100, ≥100–200 or ≥200 IU/ml and total group) according to age in a physical examination population. P<0.05, ≥10–100 IU/ml group vs. ≥100–200 group, ≥10–100 IU/ml group vs. ≥200 IU/ml group and ≥10–100 IU/ml group vs. ≥200 IU/ml group. (C) Prevalence of HBV DNA positivity in the low-level HBsAg group according to age in a physical examination population. P<0.05, enrichment method group vs. routine method group. (D) Prevalence of HBV DNA positivity in the high-level HBsAg group (≥10-100, ≥100–200 or ≥200 IU/ml, and total group) according to age in a physical examination population. P<0.05, ≥10–100 IU/ml group vs. ≥100–200 group, ≥10–100 IU/ml group vs. ≥200 IU/ml group and ≥10–100 IU/ml group vs. ≥200 IU/ml group. HBV, hepatitis B virus; HBsAg, hepatitis B surface antigen; y, years.

Article Snippet: A total of 100 μl of eluent (i.e., HBV DNA extract) was collected for real-time quantitative fluorometric detection of HBV DNA, according to the manufacturer's protocol of HBV DNA fluorescence quantitative detection reagent kit [ACON Biotech (Hangzhou) co., Ltd.] and an ABI StepOnePlusTM real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.).

Techniques: Virus

Journal: Experimental and Therapeutic Medicine

Article Title: Clinical characteristics and association analysis of persistent low-level HBsAg expression in a physical examination population with HBV infection

doi: 10.3892/etm.2019.8217

Figure Lengend Snippet: Distribution of serological patterns and clinical types in 2,544 subjects with chronic HBV infection in two groups.

Article Snippet: A total of 100 μl of eluent (i.e., HBV DNA extract) was collected for real-time quantitative fluorometric detection of HBV DNA, according to the manufacturer's protocol of HBV DNA fluorescence quantitative detection reagent kit [ACON Biotech (Hangzhou) co., Ltd.] and an ABI StepOnePlusTM real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.).

Techniques: Infection

Journal: Experimental and Therapeutic Medicine

Article Title: Clinical characteristics and association analysis of persistent low-level HBsAg expression in a physical examination population with HBV infection

doi: 10.3892/etm.2019.8217

Figure Lengend Snippet: HBV DNA and HBV markers for major serological patterns and clinical types in the two groups a .

Article Snippet: A total of 100 μl of eluent (i.e., HBV DNA extract) was collected for real-time quantitative fluorometric detection of HBV DNA, according to the manufacturer's protocol of HBV DNA fluorescence quantitative detection reagent kit [ACON Biotech (Hangzhou) co., Ltd.] and an ABI StepOnePlusTM real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.).

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Clinical characteristics and association analysis of persistent low-level HBsAg expression in a physical examination population with HBV infection

doi: 10.3892/etm.2019.8217

Figure Lengend Snippet: Analysis of the association of HBV DNA with age and HBV markers of the serological pattern HBV-M2 a , b .

Article Snippet: A total of 100 μl of eluent (i.e., HBV DNA extract) was collected for real-time quantitative fluorometric detection of HBV DNA, according to the manufacturer's protocol of HBV DNA fluorescence quantitative detection reagent kit [ACON Biotech (Hangzhou) co., Ltd.] and an ABI StepOnePlusTM real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.).

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Clinical characteristics and association analysis of persistent low-level HBsAg expression in a physical examination population with HBV infection

doi: 10.3892/etm.2019.8217

Figure Lengend Snippet: Clinical laboratory parameters for ASC patients.

Article Snippet: A total of 100 μl of eluent (i.e., HBV DNA extract) was collected for real-time quantitative fluorometric detection of HBV DNA, according to the manufacturer's protocol of HBV DNA fluorescence quantitative detection reagent kit [ACON Biotech (Hangzhou) co., Ltd.] and an ABI StepOnePlusTM real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.).

Techniques: